To further probe molecular mechanisms potentially underlying hypersensitivity of Rac1cKI myocytes to hyperactive PKA signaling, we performed bulk RNA-seq on hearts of Rac1cKI and control mice infused with saline or AngII for 3 days (Figure 8A), prior to significant genotypic differences in cardiac hypertrophy (Figure 8, B and C), or for 14 days when Rac1cKI mice have significantly more cardiac hypertrophy in response to AngII (Figure 3, A–C, and Figure 8A). This evidence concerns the gene AGT and cardiac hypertrophy.