For example, of the 400 antiplasmodial compounds in the “Malaria Box” tested for their effects on pHcyt at 1 μM only 28 caused a cytosolic alkalinization, and all of them also displayed other evidence for PfATP4 inhibition, making the assay relatively specific for ATP4 inhibitors.,, Representatives from the pyrroloacetamide and indoloacetamide cores 1–3 were tested in this assay on P. falciparum 3D7 parasites alongside the known PfATP4 inhibitor KAE609 as a positive control and the DHODH inhibitor DSM265 as a negative control. Here, DHODH is linked to malaria.