Quantitative real-time PCR (qRT-PCR) analysis showed that overexpression of Sec10 significantly attenuated the transcriptional levels of antiviral genes such as Ifn-β, Isg15, Isg54, and Isg56 induced by transfection with poly(I:C) or by vesicular stomatitis virus (VSV) infection (Fig 1A). The gene discussed is ISG15; the disease is infection.