SAMHD1 and HIV-1 infection: While HIV-1 infection of myeloid cells is antagonized by SAMHD1 [61,65–69], iMGs expressed lower levels of antiviral (dephosphorylated) SAMHD1 compared to primary MDMs (S2A Fig) and were permissive to both Lai/YU-2env (Figs 3C and S2B) and Lai∆envGFP/G (S2C Fig), and did not require co-infection with Vpx-VLPs or supplementation with excess dNs to boost infection.