To confirm the requirement of cytoplasmic HIV-1 icRNA sensing for NLRP1- inflammasome activation, we infected parental and THP1/PMA/NLRP1 macrophages with Lai∆envGFP/G WT, M10, or ∆Gag-Pol (PR-deficient) viruses (MOI = 1) and observed relatively similar levels of infection between all three viruses when analyzed by flow cytometry (Fig 6D). Here, NLRP1 is linked to infection.