Based on the mediation model adjusted for age, sex, race, poverty-income-ratio, insulin use, marital status, hypertension, total cholesterol, plasma urea nitrogen, serum albumin, and smoking status, the total effect of sedentary behavior at WWI on CVD in diabetes patients was significant (total effect, 0.0067, 95% CI, 0.003–0.008; P < 0.001). This evidence concerns the gene INS and Hypertension.