FH FOLR1-CART activation was additionally confirmed by measurement of proinflammatory cytokines IL-2, IFN-γ, and TNFα detected in 1:1 co-culture supernatant collected at 24 hours with no or minimal cytokines detected in the supernatant from NT T cells co-cultured with tumor cells (Fig. 2D). This evidence concerns the gene IL2 and neoplasm.