NF2 and hepatocellular carcinoma: Using a cutoff of more than 10‐fold‐change in enrichment and the top 50 candidate genes identified by the MAGeCK algorithm,[16] with negative controls (non‐targeting sgRNAs) included in the library design, we integrated data from 6 repetitions and identified 19 high‐hit sgRNA‐targeted genes, including the known HCC TSGs CSK[17] and NF2[18] (Figure 1F).