To validate whether RELA deficiency facilitates the transformation of primary humanized hepatocytes (PHHs) into HCC with MYC and TP53R249S overexpression in situ, we transduced PHHs with a mixture of lentivirus overexpressing MYC and TP53R249S, along with either a pair of non‐targeting‐sgRNAs (MTC) or RELA‐sgRNAs coupled with Cas9 (MTR). The gene discussed is RELA; the disease is hepatocellular carcinoma.