qPCR results revealed an induction on the transcription of solute carrier family 2 member 1 (SLC2A1), HK2, and PFKFB3 genes by G1896A mutation, and western blotting results showed that PFKFB3 was remarkably induced by G1896A mutation in HCC cells (Figure 4A,B). This evidence concerns the gene HK2 and hepatocellular carcinoma.