To investigate the effects of 8-Nitrotryp across diverse CRC biology, we selected two well-characterized cell lines representing distinct molecular subtypes: HCT116 (MSI-H, KRASG13D, TP53 wild-type) exhibits rapid proliferation and represents MSI-H tumors with defective mismatch repair; SW480 (MSS, KRASG12V, TP53 mutant) displays strong migratory and invasive behavior, characteristic of aggressive MSS subtypes often associated with KRAS mutations and EMT. This evidence concerns the gene TP53 and Marinesco-Sjogren syndrome.