P2RX7 and Anxiety: Electroacupuncture was found to attenuate nociceptive behaviors in SNI rats through suppressing synaptotagmin-1 (Syt-1) protein expression in the spinal cord.48 In addition, EA was evidenced to diminish P2X7 receptor expression that is involved in the improvement of neurobehavioral performance in SNL rats.50 As well, ALF has been reported to inhibit stress-induced hyperalgesia and anxiety sensitivity in mice.52 Based on previous literature, our findings suggest that EA and ALF could act synergically to improve antinociceptive outcomes in NP.