Immunohistochemical picture analysis showed that the expression levels of the four factors, CX3CR1, IRAK3, JUNB, and TNFR1, were significantly different between the T2DM & MAFLD groups and the Control group (P < 0.05); and the T2DM combined with MAFLD group were significantly higher than the Control group. This evidence concerns the gene CX3CR1 and type 2 diabetes mellitus.