To explore the biological function of NEURL3 in ESCC, firstly, we detected the expression of NEURL3 in several ESCC cell lines including KYSE30, KYSE140, KYSE150 and KYSE510 by using RT-qPCR and Western blotting, and the results showed that NEURL3 was highly expressed in both the KYSE30 and KYSE150 cells (Supplementary Figures S9B, C). The gene discussed is NEURL3; the disease is esophageal squamous cell carcinoma.