As shown in Figures 1E, F, there were 5 genes (CCNF, APP, NEURL3, CCNE1, AURKA) up-regulated, and 6 genes (FBXW5, TRIM13, NBR1, ANXA11, MAPK14, and CFTR) down-regulated in the ESCC group when compared to the normal group in GSE53624. Here, APP is linked to esophageal squamous cell carcinoma.