Besides, the culture supernatant of CIRT-treated HGC27 and AGS cells also upregulated the secretions or expressions of TNF-α, IL-6, IL-1β, and NOS2 in the macrophages in a dose-dependent manner (p < 0.01, Figures 2E–H), further demonstrating that CIRT had the ability to induce the M0 macrophage polarization into M1 macrophages in gastric cancer. This evidence concerns the gene NOS2 and gastric cancer.