However, the effects of SIRT1 can change depending on the biological context, so caution is needed, as excessive activation could be detrimental.68 Future research must prioritize elucidating the precise molecular links and conducting clinical trials to optimize strategies for targeting the SIRT1/NAD+ pathway, aiming to safely treat or prevent age-related neurodegenerative and cardiovascular disorders associated with circadian disruption (Fig. 5). Here, SIRT1 is linked to cardiovascular disorder.