Cancer tissues with positive expression of RUNX3 protein have strong anticancer effects, leading to the depletion of immune cells and a decrease in the number of immune cells, which provides a scientific explanation for better prognosis of RUNX3 overexpression in our previously reported study.34 As shown in Fig. 4c, both types of cancer tissues demonstrated a decrease in the number of T cells and MPs, indicating that the decrease in T cells and MPs may be a manifestation of the anticancer response in human cancer. This evidence concerns the gene RUNX3 and cancer.