We measured the relative cyst area for all germline cell types, including spermatogonial cells (SPG), primary spermatocytes (PS), metaphase I/II (MPI/II), secondary spermatocytes (SS), round spermatids (ST) and spermatozoa (SZ) and found a complete absence of round spermatids and spermatozoa (Figs 6F and 6G and S7) indicating that the primary defect in spermatogenesis in the sycp1-/- mutant is not transit through meiosis I prophase, but instead by exiting metaphase I or metaphase II. Here, SYCP1 is linked to cyst.