There is literature (9) showing that HS anemia caused by SLC4A1 gene mutation is mild, and these patients will not see a doctor in childhood, but if it is a study in an adult center, these patients need further analysis, so we did not compare the association between the age of onset of children and the type of gene mutation.The sample size in this study was relatively small, and further expansion of the sample size and collection of patient data prior to blood transfusion and splenectomy are needed for statistical analysis. This evidence concerns the gene SLC4A1 and anemia (phenotype).