NOS3 and neurodevelopmental disorder: This study used zebrafish as a model and integrated in silico toxicology, molecular docking, and RT-qPCR techniques to reveal that BPE can induce neurodevelopmental disorders and motor deficits in larvae through pathways such as inhibiting the expression of 5-HT receptors, reducing signal transduction efficiency, disrupting the cGMP/PKG pathway (with the mechanism being the reduction in NOS3/PKG stability), and promoting apoptosis.