The gating strategy and representative dot plots are shown in Fig. 3A. Quantitative analysis revealed an increase in the percentage of neutrophils (Ly6G + cells) among viable CD45 + positive cells in the spleen in 15–16-month-old male (n = 16) and female (n = 9) 3xTg-AD mice compared with age-matched controls (males n = 12; females n = 4) (Fig. 3B). The gene discussed is PTPRC; the disease is Alzheimer disease.