For instances, single-cell sequencing analysis of head and neck cancer patients’ tissues identify precursor exhausted T cells (Tpex) with stem cell characteristics positioned near DCs in uninvolved LNs, which could differentiate into intermediate exhausted T cells (Tex-int) and terminal exhausted cells (Tex) post anti-PD-L1 immunotherapy and infiltrate tumor. This evidence concerns the gene CD274 and neoplasm.