CD4 and tuberculosis: Therefore, in the present study, we sought to evaluate the phenotype profile (HLA-DR, CD-38, Ki-67) of M.tuberculosis specific CD4 + T cells producing dual IFN-γ and TNF-α cytokines from smear negative PTB patients before, during, and after completed anti-TB treatment in comparison patients with smear positive PTB, non TB other respiratory symptoms, apparently healthy QFT positive and negative study participants using polychromatic flow cytometry in Addis Ababa, Ethiopia.