We then focused on characterizing T-DARPins A2, B1, and D4, as they showed the strongest binding to TBXT (Fig. 2A), displaced TBXT from DNA in chordoma cells within 24 hours (Fig. 1E), and may act via different modes as seen from the EMSA (D4 versus A2 and B1; Fig. 1C). The gene discussed is TBXT; the disease is chordoma.