CSF significantly inhibited the fluorescent expression of TLR4, MyD88, p‐IκBα (S32/S36), and p‐p65 (S536) in lung tissues of mice with ALI (Figure 3M,N), as well as decreased the gene levels of inflammatory mediators, such as COX2, iNOS, MCP‐1, ICAM1, F4/80, CD68, IL27, and VCAM1 (Figure 3O–V). The gene discussed is TLR4; the disease is acute respiratory distress syndrome.