Given the critical role of NF‐κB p65 and NLRP3 inflammasome in ALI pathogenesis (Gross et al. 2018; Grailer et al. 2014), we examined whether the key active ingredients identified in CSF samples bind stably to NF‐κB p65 or NLRP3 and thus exert their beneficial inhibitory effects and therapeutic implications. The gene discussed is NFKB1; the disease is acute respiratory distress syndrome.