To further validate the expression of the 12 genes in clinical samples, PCR analysis was performed; the results revealed that AGER, ALDOA, FCGR2B, FTH1, HDAC7, HDAC10, ITGA2B, NCF1, NCF4, PLCB2, and PLCG1 were downregulated and that PSMA1 was upregulated in PCa tissues, which was consistent with the results of the bioinformatics analysis (Supplementary Figure S3). This evidence concerns the gene AGER and posterior cortical atrophy.