Furthermore, metabolites such as 6-oxopiperidine-2-carboxylic acid, 2, 3-dihydroxybenzoic acid, L-carnitine, decanedioic acid, and (3S, 5R, 6R, 7E)-3, 5, 6-trihydroxy-7-megastigmen-9-one showed significant positive correlations with SCC, MDA, and MPO, but negative correlations with milk yield, fat, lactose, and IgG (P < 0.05), implicating their roles in inflammatory responses, oxidative stress, and immune dysfunction. This evidence concerns the gene MPO and immune system disorder.