In some BRAF V600E mutant cell lines (DBTRG, B76, NGT41, and MAF794), we observed ERK activity is independent of upstream RTK signaling as evidenced by no signaling change in the presence or absence of serum (Supplementary Figure S1A-D), which is most consistent with prior publications characterizing BRAF V600E mutant cancers.7,8 In one line (NMCG1), we observed serum-dependence of both ERK and PI3K signaling (pERK, pS6, pAKT), indicating dependence on growth factors and relatively less dependence on BRAF V600E. Here, EIF2AK3 is linked to cancer.