Similarly, CAR-iNKT cells precultured with CD19+CD133+ CRISPRKMT2A-AFF1 ALL blasts upregulated NKG2D (supplemental Figure 5E), and subsequently were able to kill CAR target–negative SEM cells in an NKG2D-dependent manner (supplemental Figure 5F). Here, PROM1 is linked to acute lymphoblastic leukemia.