Flow cytometry analysis showed a significantly higher level of cytotoxic CD8+ T cells (Figure 2A, p < 0.001) and helper CD4+ T cells (p < 0.05) (Figure 2B) infiltration in the B16‐OVA model at both tumour stages (250 and 1000 mm3) compared to other models. The gene discussed is CD4; the disease is neoplasm.