Furthermore, high-speed video microscopy demonstrated a significant reduction in ciliary beat frequency (CBF) in the KO mice compared to the WT mice, suggestive of a phenotypic recapitulation of patients with PCD, thus confirming the impairment of ciliary motility due to DNAH10 deletion (Fig. 4E, F; Supplementary movie 1–2). This evidence concerns the gene DNAH10 and primary ciliary dyskinesia.