Based on these findings, we administered STING agonist ADU-S100 (cGAMP analog) and LTβR agonistic antibody (4H8) to tumor-bearing mice to examine whether activation of STING and LTβR pathways will induce TLS formation in TLS-free tumors by reproducing the microenvironment of TLS-rich tumors. This evidence concerns the gene STING1 and neoplasm.