We exposed the TIL products for 6–7 h to autologous tumor digest, and we measured the expression of the activation marker CD137 (4-1BB) indicative of TCR triggering (Ye et al, 2014), the degranulation marker CD107a, and TNF and IFNγ required for effective antitumoral responses (Patel et al, 2017) (Fig 3A). This evidence concerns the gene TNF and neoplasm.