Interestingly, at low E:T ratios (2.4:1), expanded MVCAR exhibited significant upregulation of human IL-2 and human IFN-γ cytokine secretions (Figures 2I and 2L) accompanied by significantly enhanced anti-tumor cytotoxicity as compared with monovalent CAR T cells (Figures 2A and 2C). This evidence concerns the gene IL2 and neoplasm.