GNB3 and depressive symptom measurement: Electroacupuncture at GV20 and LR3 alleviates depression through RNA sequencing technology, which identified changes in gene expression in the medial prefrontal cortex—including genes such as Casr, Bdkrb2, Gnb3, and Ccl1—suggesting that electroacupuncture modulates multiple biological pathways involved in the treatment of depression (93).