Capitalizing on these technical advantages, this study employed LEGENDplexTM Human Th Cytokine Panel to simultaneously quantify 12 T cell subset-associated cytokines (including IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, TNF-α) in newly diagnosed GD hyperthyroidism patients stratified by WHO iodine status criteria. Here, TNF is linked to hyperthyroidism.