In conclusion, employing a bidirectional regulatory strategy (siRNA-mediated silencing and recombinant protein rescue), we demonstrate that RPL11 inhibits the cell proliferation of renal tubular cells, induces their apoptosis, and promotes the transcription and release of inflammatory factors, exacerbating the deterioration of the inflammatory microenvironment and accelerating AKI pathogenesis. The gene discussed is RPL11; the disease is acute kidney injury.