HT29 and SW480 cells were treated with curcumin and/or doxycycline (DOX), and their cell viability, colony‐forming ability, migration, and invasion were assessed. Additionally, genes and proteins related to yes‐associated protein 1 (YAP) and PDZ‐binding motif (TAZ) signaling were analyzed via reverse transcription quantitative real‐time PCR (RT‐qPCR), western blotting, and immunofluorescence. A nude mouse xenograft tumor model was then established, and YAP and Ki67 expression levels were evaluated using immunohistochemistry (IHC) staining. The gene discussed is YAP1; the disease is neoplasm.