To further demonstrate that GSDME is the executioner protein for FGFC1-induced pyroptosis in Ara-C-resistant AML cells, we utilized the CRISPR-Cas9 system to knock down GSDME in AML Ara-C-resistant HL-60-R and K562-R cell lines (Figure 4B). Here, GSDME is linked to acute myeloid leukemia.