Therefore, in this study, we aimed to explore whether CMBs assisted by UTMD could achieve cardiac-targeted gene delivery in a porcine MI/R injury model and to evaluate whether co-administration of exogenous human SIRT3 (hSIRT3) and TIMP3 (hTIMP3) could exert myocardial protective functions. Here, SIRT3 is linked to myocardial infarction.