Furthermore, mice in the M-GFAP-hM3Dq-EA group displayed anxiety-like behaviors in the OF, EPM, and NSF compared to the M-GFAP-mCherry-EA group, including decreased time spent, number of entries, and distance in the center area in the OF (Fig. 6D–I), decreased spent time and number of entries in the EPM (Fig. 6J–M), increased latency to food in the NSF (Fig. 6N–O), and increased anxiety index in the OF and EPM (Fig. 6I, M). The gene discussed is GFAP; the disease is Anxiety.