DO analysis found that a total of 48 HS component target genes were enriched in CRC diseases, including PTGS2, DPP4, MAPK14, GSK3B, ACHE, CCND1, TNF, BAX, BCL2, CASP3, CASP8, etc. GO analysis showed that the target genes of HS components were mainly enriched in xenobiotic stimulus and lipopolysaccharide reaction. The gene discussed is TNF; the disease is colorectal carcinoma.