Among them, several genes have significant effects on the survival prognosis and TMN stage of COAD, such as NTN5, PPP1R1A, EFHD1, EME1, GLDN, CYP4F12, C1R1.AS1, VWCE, TRPM5, and TLR6. The gene discussed is GLDN; the disease is colon adenocarcinoma.