A previous study focused on lymph node metastatic tumor cells, which are highly enriched in the immune response, epithelial‒mesenchymal transition (EMT), inflammation, or hypoxia pathway-related molecules.50 We determined whether the regulation of ALDOA and ACLY activities can affect changes in characteristic genes in metastatic ESCC cells, including AXL (immune response and inflammation pathways), FGF2 and VEGFC (EMT), GALK1 and SDC2 (hypoxia), ITGA5 and MMP14 (EMT and inflammation pathway), HIF1A (inflammation), VEGFA (hypoxia and EMT), and TWIST1 (the classic marker of EMT). This evidence concerns the gene ACLY and esophageal squamous cell carcinoma.