Other studies have focused mostly on tyrosine phosphorylation enhancing the enzymatic activity of metabolic molecules.44,66,67 In the present study, we demonstrated that the phosphorylation of specific tyrosines in ACLY and ALDOA effectively enhances the enzymatic activity of these two proteins to produce OAA and G3P, which effectively facilitates the function of CDK7/9 in primary ESCC cells and c-Myc, Sox2, and Oct4 in metastatic ESCC cells. This evidence concerns the gene ACLY and esophageal squamous cell carcinoma.