We assessed the effects of the ALDOA Y174A, Y302A, Y328A, and ACLY Y542A mutants on downstream tumor-promoting and DNA replication molecules, including MCM3, MCM4, MCM5, MCM6, MCM7, CDC45, IL6, IL11, CCND1, CCNE1, CDK2, and CDK6, in primary ESCC cells (Fig. 4a).45–48 The levels of these genes were lower in primary ESCC cells transfected with ACLY Y542A than in nontransfected control cells (Fig. 4a). The gene discussed is CCNE1; the disease is esophageal squamous cell carcinoma.