Primary and metastatic ESCC cells were treated with VS-6063, VS-4718 (2.5 μM), ponatinib (5 μM), or dasatinib (0.5 μM), and the enzymatic activities of ACLY and ALDOA and relevant products, including oxaloacetate (OAA, produced by ACLY) and glyceraldehyde 3-phosphate (G3P, produced by ALDOA), were measured. Here, ACLY is linked to esophageal squamous cell carcinoma.