TNNI3 and myocardial ischemia: The most important findings of our study is that in a controlled experimental setting (i) PreC, PerC, and PostC all significantly reduce histological infarct size and cTnI release after myocardial ischemia–reperfusion, with the most pronounced reduction in infarct size observed with PreC and the least pronounced effect observed with PostC; and (ii) there were no additive effect of PostC to PerC or PostC and PerC to PreC suggesting a potential ceiling effect when combining conditioning types.