Transcriptomic analysis revealed strong upregulation of pathways related to interferon (IFN) signaling, TNFα signaling and adhesion molecules in NStat3−/− TANs, whereas tumor-promoting pathways, e.g., extracellular matrix remodeling, angiogenesis, immune checkpoint and immune suppression, were downregulated (Fig. 3e). Here, TNF is linked to neoplasm.