KCNK2 and brain ischemia: Additionally, TREK-1 activity is important for cardiomyocyte response to pressure overload,194 pain perception,186 brain ischemia,195 and morphine analgesia.196 Despite TREK-1 undergoing alternative splicing in both neurons and the heart, much remains unknown about the roles of TREK-1 isoforms in modulating TREK-1 activity in cardiovascular disease and in the central and peripheral nervous systems in vivo.