We found that in the late stages of the tumor, EMT and FAM in tumor cells increased significantly, and their spatial distribution was consistent with that of metastatic tumors, suggesting that the upregulation of FAM may be closely related to lymph node metastasis and that the evolution from the initial stage to the disseminated stage is parallel to the tumor metabolic pathway (Figure 4D, Supplementary Figure S4). Here, USP9X is linked to metastatic neoplasm.