DLG3 and cancer: Based on the comprehensive analysis of bulk transcriptome, single-cell transcriptome and spatial transcriptome, we noticed that DLG3 was not only negatively correlated with CD8T cells and positively correlated with M2 macrophages, but also associated with several indicators of immunotherapy (inflammatory T cells, cancer immune cycle, etc.), and the subsequent multi-cohort analysis confirmed that DLG3 had some predictive potential for immunotherapy.