ACTN1 and type 2 diabetes mellitus: For instance, out of eight methylation sites in the Actinin Alpha 1 (ACTN1)gene, higher methylation levels at cg01847441 and cg11724590 were negatively associated with the risk of T2DM, while higher methylation levels at cg00841968, cg09421468, cg13577778, cg13693582, cg20056076, and cg23052861 were positively associated with the risk of T2DM (Table S2 in the Online Supplementary Document).