To this end, using published methods (3), we generated an expression construct with the NAGLU gene encoding α-N-acetylglucosaminidase (the enzyme deficient in MPS IIIB), a 6-glycine linker, and a 114-bp nucleotide segment corresponding to the transmembrane region and cytosolic tail of LAMP1, which encodes the lysosomal-associated membrane protein-1, followed by a c-Myc epitope tag after the cytosolic tail. Here, LAMP1 is linked to mucopolysaccharidosis type 3B.