Strikingly, regain of TLR4 in myocytes of the Cre-recombinase injected leg in ID;Tlr4Myo;r-mS100A9 mice failed to improve hyperglycemia and increase glucose uptake (Fig. 5B and 5C) indicating that myocyte TLR4 is dispensable for S100A9's glucoregulatory effects. Here, TLR4 is linked to Hyperglycemia.