After characterizing the diffusion behavior of naked DNA origami,we aimed at investigating the effect of ligand functionalization.We selected the anti-EGFR antibody as a functional cargo due to itsrelevance in colorectal cancer therapeutics, where its delivery acrossmucus is highly desirable. To this end,anti-EGFR was conjugated to an ssDNA oligonucleotide using a click-chemistryapproach. This evidence concerns the gene EGFR and colorectal cancer.